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In the human nervous system, available studies assert; that the Neuroglia cells (special neurons in the brain) may provide structural support and/or regenerative tendencies (gliosis) to damages within the circuitry. This study conducted to investigate the activity of Ocimum gratissimum leaf extract on the regenerative functions of the cerebellum of lead II acetate exposed wistar rats. A total of twenty five (25) Wistar rats were divided into five (5) groups of five (5) rats each. With Group one (1) receiving standard rat diet and water only (control group), Groups 2, 3, 4 and 5 respectively got 252.98 mg/kg/day of aqueous O. gratissimum leaves extract for 35 days, 180 mg/kg of lead II acetate for 21 days, 180 mg/kg of lead II acetate for 21 days with continued treatment of 126.49 mg/kg of O. gratissimum leaf extract for 35 days, and 180 mg/kg of lead II acetate with continued treatment of 252.98 mg/kg of O. gratissimum leaf extract for 21 days. Rats were then euthanized (after treatment period), with blood samples obtained and assayed for biochemical changes. Histological and stereological examinations were also conducted for cerebella tissue. Upon comparison with control (group I), one-way analysis of variance (ANOVA) returned a significantly high GSH value for group one rats as compared to other groups. Even though huge degeneration was seen for group III rats, Cerebella Malonaldehyde, Superoxide Dismutase, Glutathione and Catalase levels, were relatively low across groups IV and V as against the control. Histological section through group I rats showed cerebella purkinje layer appearing deeply basophilic, with a characteristically condensed Nissl granule, thus describing a normal architecture of the cerebellar cortex. Pyramidal and granular axonic and dendritic cells were also seen to have embedded neuropils within group II rats. Conclusively, Study has shown that O. gratissimum extract consumption may be potent for low to minimal anti-oxidant effect, and regenerative tendencies (gliosis) on the cerebellum. More sophisticated but similar studies are recommended for other areas of the brain.